| ORIGINAL ARTICLE |
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| Year : 2015 | Volume
: 4
| Issue : 1 | Page : 13-18 |
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Evaluation of the cytotoxicity of fiber reinforced composite bonded retainers and flexible spiral wires retainers in simulated high and low cariogenic environments
Arezoo Jahanbin1, Mostafa Shahabi1, Farzaneh Ahrari1, Yasaman Bozorgnia2, Arezoo Shajiei3, Hooman Shafaee1, Jalil Tavakkol Afshari4
1 Dental Research Center, Department of Orthodontics, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
2 Department of Orthodontics, School of Dentistry, Bojnord University of Medical Sciences, Bojnord, Iran
3 Immunology Research Center, Immunogenetic and Tissue Culture Department, Mashhad University of Medical Sciences, Mashhad, Iran
4 Bu Ali Research Institute, Immunogenetic and Tissue Culture Department, Mashhad University of Medical Sciences, Mashhad, Iran
Correspondence Address:
Hooman Shafaee
Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Vakil Abad Boulevard, Mashhad
Iran
 Source of Support: This research was financially supported
by research vice chancellor of Mashhad University of Medical
Sciences,, Conflict of Interest: None  | Check |
DOI: 10.4103/2278-0203.149610
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Objectives: The aim of this study was to evaluate the cytotoxic effects of fiber reinforced composite bonded retainers in comparison with flexible spiral wires (FSWs) under high and low cariogenic-simulated environments using human oral fibroblasts.
Materials and Methods: Four types of bonded retainers were evaluated: (1) reinforced with glass fibers: Interlig (Angelus), (2) reinforced with polyethylene fibers: Connect (Kerr), (3) reinforced with quartz fibers: Quartz Splint UD (RTD), and (4) FSW. Twenty specimens of each sample group were prepared with the same surface area and halved. Next, half of them were placed in a high cariogenic environment 60 min in 10% lactic acid 3 times a day and remained in Fusayama Meyer artificial saliva for the rest of the day) and the other half were placed in a low cariogenic environment 20 min in 10% lactic acid 3 times a day and remained in Fusayama Meyer artificial saliva for the rest of the day) for 1, 7 and 30 days. Cell viability was assessed by MTT assay. Data were analyzed using SPSS software (α =0.05).
Results: During the 1 st month, cytotoxicity reduced gradually. In the low cariogenic-simulated environment, the cytotoxicity of all of the groups were reported to be mild at day 30 and the difference between them was significant (P = 0.016). In the same period in the high cariogenic-simulated environment, the cytotoxicity of Connect and Quartz Splint was mild, and they had lower cytotoxicity than the other groups. Meanwhile, Interlig had moderate (52%) and FSW had severe cytotoxicity (22%) and the difference between the groups was also significant (P = 0.000).
Conclusions: FSW retainers are not recommended in those at high-risk for dental caries. However, in those at low-risk, there is no difference from the standpoint of cytotoxicity. |
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